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The effect of starch and ineubation temperature in anther culture of potato

✍ Scribed by Eva K. Calleberg; Lars B. Johansson

Publisher: Springer
Year: 1993
Tongue: English
Weight: 553 KB
Volume: 32
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00040112

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✦ Synopsis

Three Andean tetraploid potato genotypes (2n= 48) and 7 anther-derived dihaploids (2n=24) originating from two of the tetraploids were used in anther culture. Relative number of embryos/vial was significantly higher when the anther culture media was gelatinized with 3% potato starch than when Gelrite or wheat starch (3%) were used as gelatinizing agents. The degree of anther culture response varied between tetraploids but also within a group of related dihaploids. Additionally, the embryo production of individual genotypes, tetraploids as well as dihaploids, was dependent on the incubation temperature (10, 15, 20, 25, 30°C) of the anther culture. The incubation temperature of the anther culture was also important for the regeneration rate. Direct regeneration was mostly stimulated when the anther culture was incubated at 20°C.

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