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Influence of 2,4-dand lactose on pollen embryogenesis in anther culture of potato

✍ Scribed by Ludmila Říhová; Jaroslav Tupý

Publisher
Springer
Year
1996
Tongue
English
Weight
258 KB
Volume
45
Category
Article
ISSN
0167-6857

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✦ Synopsis

Anthers of diploid genotypes of Solanum tuberosum capable of androgenesis were cultured on different media to examine the effect on induction of pollen embryogenesis of 2,4-D and lactose. Anthers cultured in callogenic medium with 2,4-D and sucrose produced pollen derived embryoids only exceptionally. When sucrose was replaced by lactose the frequency of embryogenesis was as high or higher than in embryogenic auxin-free medium. Substitution of lactose for sucrose in the embryogenic medium had no effect. Supplementing the embryogenic medium with 2,4-0 strongly reduced the frequency of pollen embryoids in the presence of sucrose but not with lactose.

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