The stability of foreign protein production in genetically modified plant cells

✍ Scribed by Jianwei Gao; James M. Lee; Gynheung An

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 407 KB
• Volume: 10
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00234589

✦ Synopsis

The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independently transformed cell lines either in the presence or the absence of selectable antibiotics. Specific GUS activity was stably maintained in five lines. About a two-to four-fold increase in the GUS activity was observed from three cell lines. The remaining two cell lines lost the activity within the first 70 to 210 days. The presence of antibiotics did not significantly alter the stability of the foreign protein production in all cell lines examined.