The solvent effect in enzyme—substrate interactions: Models of carboxypeptidase
✍ Scribed by Carol A. Venanzi; Harel Weinstein; Georgina Corongiu; Enrico Clementi
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 530 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0020-7608
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Milk lactose is hydrolysed to D-galactose and D-glucose in the small intestine of mammals by the iactase-phlorizin hydrolase complex (LPH,. Lactase activity has broad substrate selectivity and several glycosides are substrates. Recently, using the monodeoxy derivatives of methyl /3-1actoside (1), we
A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme prepara