A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme prepara
Influence of organic solvents on enzyme chemoselectivity and their role in enzyme-substrate interaction
β Scribed by Cynthia Ebert; Lucia Gardossi; Paolo Linda; Regina Vesnaver; Marco Bosco
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- French
- Weight
- 570 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0040-4020
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π SIMILAR VOLUMES
The enantioselectivities of subtilisin Carlsberg and Rhizomucor miehei lipase in organic solvents are found to strongly depend on the method by which the enzymes are prepared. For the transesterification between sec- phenethyl alcohol and vinyl butyrate in dioxane at 7"C, the enantioselectivity of s
Catalytic activities of β£-chymotrypsin and subtilisin Carlsberg in various hydrous organic solvents were measured as a function of how the enzyme suspension had been prepared. In one method, lyophilized enzyme was directly suspended in the solvent containing 1% water. In another, the enzyme was prec
## Abstract For Abstract see ChemInform Abstract in Full Text.