The solubilizing properties of the protein-detergent complex
β Scribed by Ira Blei
- Publisher
- Elsevier Science
- Year
- 1959
- Weight
- 198 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0095-8522
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Working with detergent-solubilized bacteriorhodopsin we have used a table top preparative centrifuge for determination of \(M_{\mathrm{r}}\) of membrane proteins by sedimentation equilibrium. We demonstrate the use of two new methods to measure protein concentration as a function of distance from ro
A procedure was developed for the quantitation of solubilized proteins using the Bradford assay in the presence of glucopyranoside detergents. These detergents solubilized membranebound proteins with minimal background absorbance at 595 nm. Absorbance at 650 nm was also low, indicating that these de