The mechanism of solubilization in detergent solutions
β Scribed by J.A Shaeiwitz; A.F-C Chan; E.L Cussler; D.F Evans
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 657 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0021-9797
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Working with detergent-solubilized bacteriorhodopsin we have used a table top preparative centrifuge for determination of \(M_{\mathrm{r}}\) of membrane proteins by sedimentation equilibrium. We demonstrate the use of two new methods to measure protein concentration as a function of distance from ro
## Abstract The HLB dependency for the solubilization of membrane proteins and adenylate cyclase activity from a plasma membraneβenriched fraction from rat liver has been determined. The HLB (hydrophilic/lipophilic/balance) number of a detergent is an empirical measure of its relative hydrophobicit