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Microsomal methionine aminopeptidase: Properties of the detergent-solubilized enzyme

✍ Scribed by Jose Olavo Freitas Jr; Carlos Termignoni; Jorge A. Guimarães

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
678 KB
Volume
17
Category
Article
ISSN
0020-711X

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A new nondenaturing zwitterion detergent has been used to solubilize the vitamin K-dependent carboxylating system from rat liver microsomes. The optimal conditions for solubilization were 1% 3-t (3-cholamidopropyl)dimethylammonio]-I -propane sulfonate (CHAPS) and 1 M NaCl while maximum carboxylating