A new detergent for the solubilization of the vitamin K-dependent carboxylation system from liver microsomes: Comparison with triton X-100
✍ Scribed by Jean-Marie Girardot; B.Connor Johnson
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 487 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A new nondenaturing zwitterion detergent has been used to solubilize the vitamin K-dependent carboxylating system from rat liver microsomes. The optimal conditions for solubilization were 1% 3-t (3-cholamidopropyl)dimethylammonio]-I -propane sulfonate (CHAPS) and 1 M NaCl while maximum carboxylating activity was achieved with 0.5% CHAPS, 0.75 M NaCl for a microsomal protein concentration varying from 7.5 to 10 mg/ml. Using CHAPS, high levels of carboxylase activity can be found not only in liver microsomes from vitamin Kdeficient rats, but also in microsomes prepared from the livers of normal animals, normal animals supplemented with vitamin K,, or vitamin K-deficient animals supplemented with vitamin K,, while little if any activity is detected after Triton X-100 solubilization of the microsomes of such livers. Thus, the new zwitterion detergent proves to be more effective and less denaturing in solubilizing the vitamin K-dependent carboxylase from rat liver microsomes than other detergents used in this system.