The separation of myo-inositol phosphates by ion-pair chromatography
β Scribed by James A. Shayman; Dawn M. BeMent
- Book ID
- 115761280
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 515 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0006-291X
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We have developed an ion-pair reverse-phase HPLC method to measure inositol phosphates in 32P-labeled cells. The different chromatographic parameters were analyzed to optimize the resolution of the 32P-labeled metabolites. Analysis of inositol phosphates in biological samples was improved by a singl
Of the ion-chromatographically distinct isomers, 11 of the 12 myo-inositol trisphosphates (IP,) and all of the 9 myo-inositol bisphosphates (IP,) have been found in a chemical hydrolysate of phytic acid. The isomers were fractionated by anion-exchange chromatography by a method that is suitable for
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