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Preparative separation of myo-inositol bis- and tris-phosphate isomers by anion-exchange chromatography

✍ Scribed by Barbara Goldschmidt


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
347 KB
Volume
208
Category
Article
ISSN
0008-6215

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✦ Synopsis


Of the ion-chromatographically distinct isomers, 11 of the 12 myo-inositol trisphosphates (IP,) and all of the 9 myo-inositol bisphosphates (IP,) have been found in a chemical hydrolysate of phytic acid. The isomers were fractionated by anion-exchange chromatography by a method that is suitable for the purification of mg amounts of the IP, and IP, isomers.


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## Abstract Phosphorylated carbohydrates are important intracellular metabolites and thus of prime interest in metabolomics research. Complications in their analysis arise from the existence of structural isomers that do have similar fragmentation patterns in MS/MS and are hard to resolve chromatog