Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
✍ Scribed by Pazourek, Jiří
- Book ID
- 122209943
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 359 KB
- Volume
- 391
- Category
- Article
- ISSN
- 0008-6215
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Of the ion-chromatographically distinct isomers, 11 of the 12 myo-inositol trisphosphates (IP,) and all of the 9 myo-inositol bisphosphates (IP,) have been found in a chemical hydrolysate of phytic acid. The isomers were fractionated by anion-exchange chromatography by a method that is suitable for
We developed a HPLC method which separates the following nine inositol-containing compounds of biological interest: inositol, inositol 1-monophosphate, inositol 2- or 4-monophosphate, inositol 1,2-cyclic phosphate, inositol 1,4-bisphosphate, inositol 1,4,5-trisphosphate, glycerophosphoinositol, glyc