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The role of the positively charged N-terminus of the signal sequence of E. coli outer membrane protein PhoE in export

✍ Scribed by Dirk Bosch; Paul de Boer; Wilbert Bitter; Jan Tommassen


Book ID
115729197
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
618 KB
Volume
979
Category
Article
ISSN
0005-2736

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To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat