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Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residues

✍ Scribed by Patrick Van Gelder; Nathalie Saint; Prashant Phale; Elaine F Eppens; Alexej Prilipov; Ria van Boxtel; Jürg P Rosenbusch; Jan Tommassen

Book ID
115627961
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
193 KB
Volume
269
Category
Article
ISSN
0022-2836

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Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12
✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat