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The ribosomal protein gene cluster of Mycoplasma capricolum

โœ Scribed by Ohkubo, Shoichi ;Muto, Akira ;Kawauchi, Yasushi ;Yamao, Fumiaki ;Osawa, Syozo


Publisher
Springer
Year
1987
Tongue
English
Weight
982 KB
Volume
210
Category
Article
ISSN
0026-8925

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Molecular cloning of ribosomal protein g
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A Bg/II-fragment from the Mycoplasma capricolum DNA cloned into pBR322 has been found to contain a cluster of ribosomal protein genes. The recombinant plasmid, pMCB1088, includes a 9 kilobase-pair insert that codes for at least eight ribosomal proteins of M. capricolum. The protein genes are express

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DNA segments carrying rRNA genes of Mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, DNA-RNA hybridization and nucleotide sequencing. The M. capricolum genome has two sets of rRNA gene clusters, where the arrangement is in the order of (5')16S-23S-5S(3').

The number of ribosomal RNA genes in Myc
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We have examined the number of rRNA genes in Mycoplasma capricolum (KID) by hybridization of Bg/II-, EcoRI- and Xbal-digests of DNA to [3'-32P] 16S, 23S and 5S rRNAs according to the Southern procedure (1975). All the restriction gels gave two radioactive bands with three kinds of rRNA. Furthermore,

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The nucleotide sequences of the rrnB 16S ribosomal RNA gene and its 5'-and 3'-flanking regions from Mycoplasma capricolum have been determined. The coding sequence is 1521 base pairs long, being 21 base pairs shorter than that of the Escherichia coli 16S rRNA gene. The 16S rRNA sequence of M. capric

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Strains of Bacillus subtilis harboring mutations causing altered migration of one or more of ten ribosomal proteins were used to map the corresponding gene loci. The loci of the genes for proteins BL1, BL5, BL9, and BL11 mapped between cysA and rpoB. The locus of the gene for protein S7 was very clo