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Organization of ribosomal RNA genes in Mycoplasma capricolum

โœ Scribed by Sawada, Makoto ;Muto, Akira ;Iwami, Masafumi ;Yamao, Fumiaki ;Osawa, Syozo

Publisher
Springer
Year
1984
Tongue
English
Weight
909 KB
Volume
196
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

DNA segments carrying rRNA genes of Mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, DNA-RNA hybridization and nucleotide sequencing. The M. capricolum genome has two sets of rRNA gene clusters, where the arrangement is in the order of (5')16S-23S-5S(3'). The spacer region between 16S and 23S rDNA is extremely rich in AT and does not carry any tRNA genes.

๐Ÿ“œ SIMILAR VOLUMES

The number of ribosomal RNA genes in Myc
The number of ribosomal RNA genes in Mycoplasma capricolum
โœ Sawada, Makoto ;Osawa, Syozo ;Kobayashi, Hideki ;Hori, Hiroshi ;Muto, Akira ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 225 KB

We have examined the number of rRNA genes in Mycoplasma capricolum (KID) by hybridization of Bg/II-, EcoRI- and Xbal-digests of DNA to [3'-32P] 16S, 23S and 5S rRNAs according to the Southern procedure (1975). All the restriction gels gave two radioactive bands with three kinds of rRNA. Furthermore,

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The nucleotide sequences of the rrnB 16S ribosomal RNA gene and its 5'-and 3'-flanking regions from Mycoplasma capricolum have been determined. The coding sequence is 1521 base pairs long, being 21 base pairs shorter than that of the Escherichia coli 16S rRNA gene. The 16S rRNA sequence of M. capric