We have studied recombination between repetitive mitochondrial DNAs from cytoplasmic petite mutants of Saccharomyces cerevisiae. Mitochondrial DNA was isolated from two parental p- mutants, carrying respectively the CR and the ER mitochondrial genetic markers, and from two p- CRER diploid genetic re
The restriction of the recombination of mitochondrial DNA molecules in the zygotes of Saccharomyces cerevisiae
β Scribed by Waxman, Michael F.
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 360 KB
- Volume
- 141
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Crosses were made between strains carrying a nuclear control factor (NC) and strains classified with respect to their omega allele (omega+ or omega-). The characteristic asymmetrical transmission was always observed (as was seen) in crosses not involving the omega factor. The analysis of functional recombinants in a cross involving an NC factor has indicated that the absence of the omega effect may be caused by a restriction in the zygote of the recombination of mitochondrial DNA molecules.
π SIMILAR VOLUMES
Mitotic cells of a diploid strain of Saccharomyces cerevisiae with appropriate markers for the detection of mitotic crossing-over and mitotic gene conversion were irradiated with X-rays. Induction of these recombinational events was strong. After irradiation, cells were incubated in a rich growth me