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Mitotic recombination in the absence of synaptonemal complexes in Saccharomyces cerevisiae

✍ Scribed by Olson, L. W. ;Zimmermann, F. K.

Publisher: Springer
Year: 1978
Tongue: English
Weight: 489 KB
Volume: 166
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00285918

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✦ Synopsis

Mitotic cells of a diploid strain of Saccharomyces cerevisiae with appropriate markers for the detection of mitotic crossing-over and mitotic gene conversion were irradiated with X-rays. Induction of these recombinational events was strong. After irradiation, cells were incubated in a rich growth medium and samples were removed for studying the possible formation of synaptonemal complexes up to a time when most cells had completed the first post-irradiation cell division. No complexes were found during the entire period of sampling, during which mitotic recombination in G1 (mitotic gene conversion), DNA replication and G2 (mitotic crossing-over) had occurred. These results are interpreted to mean that synaptonemal complexes are not required for mitotic recombination.

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