The resolution of polyoma DNA by polyacrylamide gel electrophoresis
β Scribed by E.H. Harley; J.S. White; E.D. Rubery
- Book ID
- 115902693
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 363 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-5793
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Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and perc
## Abstract Polyacrylamide gel electrophoresis (PAGE) is routinely used for electrophoretic characterization of biomolecules. In this study we demonstrate that this technique is quite useful for the characterization of small molecules, however, it becomes inadequate for the resolution of highβmolec