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Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking

✍ Scribed by George Johnson

Book ID
104787357
Publisher
Springer
Year
1979
Tongue
English
Weight
807 KB
Volume
17
Category
Article
ISSN
0006-2928

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✦ Synopsis

Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and percent crosslinking within a single linear regression alaysis.

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## Abstract Polyacrylamide gel electrophoresis (PAGE) is routinely used for electrophoretic characterization of biomolecules. In this study we demonstrate that this technique is quite useful for the characterization of small molecules, however, it becomes inadequate for the resolution of high‐molec