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The requirement of DNA synthesis for the induction of alkaline phosphatase by bromodeoxyuridine in a derivative of the HeLa cell line

✍ Scribed by John Morrow; Douglas M. Stocco; Joe A. Fralick

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 722 KB
Volume: 98
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040980218

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Non‐lethal concentrations of bromodeoxyuridine induce a 2‐ to 5‐fold increase in the specific activity of alkaline phosphatase in HeLa subclone, S3G. Experiments employing 10‐hour pulses of BRdU showed that 48 hours were required before induction commenced, and that maximal induction was attained by 96 hours. Under conditions in which DNA synthesis was prevented with hydroxyurea induction did not occur. Upon removal of hydroxyurea both DNA synthesis and induction were rapidly reestablished. Furthermore, experiments employing radiolabelled BRdU demonstrated that the kinetics of the induction process paralleled the incorporation of the analogue into cellular DNA.

These results indicate that DNA synthesis, or some process intimately linked to DNA synthesis, is required for the induction of alkaline phosphatase, and suggest that the mode of the induction may be through the incorporation of the analogue into cellular DNA.

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