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Recombinant human interleukin-1 inhibits the induction by dexamethasone of alkaline phosphatase activity in murine capillary endothelial cells

✍ Scribed by Mary A. Mulkins; Anthony C. Allison

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
645 KB
Volume
133
Category
Article
ISSN
0021-9541

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✦ Synopsis

A mutual antagonism exists between interleukin-Is (IL-Is) as pro-inflammatory and glucocorticoids as anti-inflammatory mediators. This report examines the effects of IL-1 on the induction by dexamethasone of alkaline phosphatase in LEI1 murine endothelial cells. Dexamethasone increases the specific activity of alkaline phosphatase in a time-and dose-dependent fashion (maximum 14fold induction at M), and this induction can be completely inhibited by simultaneous incubation with picomolar concentrations of recombinant human IL-la or IL-10. This IL-I-mediated antagonism of dexamethasone activity is not due to a down-regulation of glucocorticoid receptors in t h e cell line used, because the number of receptors and their affinity for dexamethasone is unchanged in IL-I-treated cells. However, induction of alkaline phosphatase by dexamethasone in LEI1 cells is receptor-mediated, since it can also be inhibited by glucocorticoid-receptor antagonists.

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