The regulatory nature of thephoBgene for alkaline phosphatase synthesis inEscherichia coli
β Scribed by Ezra Yagil; Moshe Bracha; Yael Lifshitz
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 361 KB
- Volume
- 137
- Category
- Article
- ISSN
- 1617-4615
No coin nor oath required. For personal study only.
β¦ Synopsis
Quantitative measurements of alkaline phosphatase activity in various merozygotic combinations and electrophoretic analyses of periplasmic proteins derepressed by phosphate-starvation show that phoB is a positive regulatory gene.
π SIMILAR VOLUMES
Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment whic
## Abstract Nonβlethal concentrations of bromodeoxyuridine induce a 2β to 5βfold increase in the specific activity of alkaline phosphatase in HeLa subclone, S3G. Experiments employing 10βhour pulses of BRdU showed that 48 hours were required before induction commenced, and that maximal induction wa