Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli

✍ Scribed by Boidol, Werner ;Simonis, Marianne ;Töpert, Michael ;Siewert, Gerhard

• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 304 KB
• Volume: 185
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00334150

✦ Synopsis

Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived.