𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis and optimization of recombinant protein production in Escherichia coli using the inducible pho A promoter of the E. coli alkaline phosphatase

✍ Scribed by Carsten Lübke; Werner Boidol; Thomas Petri


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
731 KB
Volume
17
Category
Article
ISSN
0141-0229

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


The effect of heating rate on Escherichi
✍ Luis Caspeta; Noemí Flores; Néstor O. Pérez; Francisco Bolívar; Octavio T. Ramír 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 270 KB 👁 2 views

## Abstract At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat‐inducible systems. However, for large scale‐cultures only slow ramp‐type increases in temperature are possible due to heat transfer limitations, where

Fed-batch cultivation of recombinant esc
✍ Lutz Brandes; Xiaoan Wu; Jens Bode; Jong II Rhee; Karl Schügerl 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

EcoRI fusion protein was produced by Escherichia coli JM103 carrying the multicopy expression plasmid pMTC48, the multicopy repressor plasmid pRK248, and the multicopy protection plasmid pEcoR4 in a 60-L working volume airlift tower loop reactor on M9 minimal medium with glucose. Cell mass concentra