Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring

Monitoring GFP-operon fusion protein exp

Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor

✍ Matthew P. DeLisa; Jincai Li; Govind Rao; William A. Weigand; William E. Bentley 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB 👁 1 views

Synthesis of an operon fusion protein was investigated in batch and fed-batch cultures at high cell densities of recombinant Escherichia coli JM105 [pBAD-GFP::CAT]. Glucose-limited growth was achieved without accumulation of inhibitory byproducts allowing high cell densities (110 g L -1 DCW) to be a