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The reaction of glutaraldehyde with tissue lipids

✍ Scribed by Roy Gigg; Sheila Payne

Publisher: Elsevier Science
Year: 1969
Tongue: English
Weight: 167 KB
Volume: 3
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(69)90021-8

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✦ Synopsis

Portions of mouse brain, ktdneF and h~er were fixed wuh g[utaraldeh~de and the hp~ds were then extracted with chloroform methanol Comparison of the extracted hplds with those from stmdar porhons of the same unfixed hssues by thin la~,er chromatography sho~ed the absence of phosphatldyl ethanolamme in the extract from the fixed tissues Actd hydrolFs~s of the fixed, extracted hssue hberated free fatt~ acMs suggesting that the phosphattdyl ethanolamme had been fixed to the tissue proteins

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