Treatment with lithium salts reduces ethanol dehydration shrinkage of glutaraldehyde fixed tissue
✍ Scribed by A. Boyde; E. Maconnachie
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 332 KB
- Volume
- 66
- Category
- Article
- ISSN
- 1432-119X
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✦ Synopsis
Changes in the area of glutaraldehyde fixed 15 day p.c. mouse embryo limbs were recorded using a Quantimet 720 image analysing computer attached to a light microscope: during a period of treatment with an isotonic salt solution (mostly halides of the alkali or alkaline earth metals); a subsequent wash with distilled water; and dehydration through a 30, 50, 70, 80, 90, and 100% ethanol series. Pretreatment with NaC1, KC1, RbC1 had no significant effect. Treatment with LiC1, LiNO3, LiF (0.03 M), CsF and CsC1 caused an increase (relative to Na, K or Rb treated samples) in the specimen volume during dehydration, which persisted in 100% ethanol. Li treated samples showed the largest post-critical-point-drying (CPD) volumes, followed by Cs treated tissue. Pretreatment with Be, Mg, Ca, Sr and Ba chlorides caused shrinkage and the 100% ethanol and post-CPD volumes of these samples were all lower than those treated with the monovalent cation containing salts.