The rapid and direct determination of ATPase activity by ion exchange chromatography and the application to the activity of heat shock protein-90
โ Scribed by Manuela Bartolini; Irving W. Wainer; Carlo Bertucci; Vincenza Andrisano
- Book ID
- 118477631
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 308 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0731-7085
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Heat shock protein 90 (Hsp90) is an abundant protein and essential for all eukaryotic cells. The expression of Hsp90 is further enhanced after exposure to stress factors, e.g. a heat shock. Many proteins interacting with Hsp90 as well as the various functions for Hsp90 have been describ
We have developed an electrospray ionisation mass spectrometry (ESI-MS) technique that can be applied to rapidly determine the number of intact active sites in proteins. The methodology relies on inhibiting the protein with an active-site irreversible inhibitor and then using ESI-MS to determine the