Intracellular localization of the 90 kDA heat shock protein (HSP90α) determined by expression of a EGFP–HSP90α-fusion protein in unstressed and heat stressed 3T3 cells
✍ Scribed by Thomas Langer; Susann Rosmus; Hugo Fasold
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 241 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
Abstract
Heat shock protein 90 (Hsp90) is an abundant protein and essential for all eukaryotic cells. The expression of Hsp90 is further enhanced after exposure to stress factors, e.g. a heat shock. Many proteins interacting with Hsp90 as well as the various functions for Hsp90 have been described. In this study, an Hsp90α fusion protein along with the enhanced green fluorescence protein (EGFP) was expressed under the control of the human cytomegalovirus immediate early promoter. EGFP—Hsp90α was mainly localized in the cytoplasm, with only minor amounts inside the nuclei. No EGFP—Hsp90α could be detected inside the nucleoli. Following exposure to elevated temperatures, higher amounts of EGFP—Hsp90α are inside the nucleus, but not within the nucleoli. As the most remarkable finding under these conditions, an association of EGFP—Hsp90α with the nuclear membrane became visible.