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The radiopurity of S-adenosylmethionine and S-adenosylethionine preparations

✍ Scribed by F. Schlenk; C. R. Zydek

Publisher
John Wiley and Sons
Year
1967
Tongue
French
Weight
409 KB
Volume
3
Category
Article
ISSN
0022-2135

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✦ Synopsis

In the biosynthesis of labeled S-adenosylmethionine and S-adenosylethionine from the corresponding 1"-labeled sulfur amino acids by yeast, some radioimpurity occurred by non-speci@ labeling of the sulfonium compounds. This is explained by partial degradation of methionine and ethionine in the yeast cells, followed by utilization of the randomized fragments in the biosynthesis. The degree of mislabeling remains low because endogenous methionine synthesis .from metabolic pool constituents is suppressed by the excess of methionine and ethionine supplement in the culture medium. Hydrolysis of the sulfonium compounds and analysis of the fragments showed that the misplaced isotope amounts to approximately 0.5 % in the adenine, and 0.2 in the homoserine part compared with the total radioactivity of the sulfonium compounds (100 %) .

πŸ“œ SIMILAR VOLUMES

Enzymatic synthesis of 11C-labelled S-ad
Enzymatic synthesis of 11C-labelled S-adenosylmethionine
✍ Paul Gueguen; Jean-Louis Morgat; Mariannick Maziere; GΓ©rard Berger; Dominique Co πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 French βš– 480 KB

## Abstract This article describes the radiosynthesis of ^11^C‐labelled S‐adenosylmethionine by an enzymatic process. This methyl donor was prepared by condensation of L‐(methyl‐^11^C) methionine with ATP. The synthesis and purification could be carried out in 20 minutes with a final yield of 80 %.