A method is described for measuring the elastin content of whole tissue. Desmosine and isodesmosine, the cross-linking amino acids unique to elasiin, were first separated by paper chromatography and then quantitated by amino acid analysis. Results compared favorably to those obtained with gravimetri
The presence in elastin of possible cyclic precursors of desmosine and isodesmosine
โ Scribed by Mercedes A. Paz; Paul M. Gallop; Olga O. Blumenfeld; Edward Henson; Sam Seifter
- Book ID
- 118852842
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 395 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the
Quantitation of desmosine and isodesmosine, the major crosslinks in elastin, has been of interest because of their uniqueness and use as markers of that protein. Accurate measurement of these crosslinks may allow determination of elastin degradation in vivo and elastin content in tissues, obviating
## Abstract Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers