We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the
Determination of the elastin content of tissues by measuring desmosine and isodesmosine
โ Scribed by Barry C. Starcher
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 294 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A method is described for measuring the elastin content of whole tissue. Desmosine and isodesmosine, the cross-linking amino acids unique to elasiin, were first separated by paper chromatography and then quantitated by amino acid analysis. Results compared favorably to those obtained with gravimetric techniques, but the method has been extended to include the quantitation of elastin from small amounts of tissues that contain low concentrations of elastin. I1
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Quantitation of desmosine and isodesmosine, the major crosslinks in elastin, has been of interest because of their uniqueness and use as markers of that protein. Accurate measurement of these crosslinks may allow determination of elastin degradation in vivo and elastin content in tissues, obviating
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