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Comparison of elisa and hplc for the determination of desmosine or isodesmosine in aortic tissue elastin

✍ Scribed by Dr. Toru Osakabe; Yoshiyuki Seyama; Saburo Yamashita

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
322 KB
Volume
9
Category
Article
ISSN
0887-8013

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✦ Synopsis

We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the two different methods, a good linear relationship was obtained (r = 0.854 for human aorta or r = 0.938 for rabbit aorta, respectively). The ELISA method can detect as little as 6 pmol/ml and it may be useful in monitoring elastin metabolism in patients with various connective tissue diseases.

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