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The preparation of [35S]homocysteine thiolactone free of [35S]methionine

✍ Scribed by Peter H. Stern; James O. Mecham; Robert M. Hoffman

Book ID
118387393
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
227 KB
Volume
7
Category
Article
ISSN
0165-022X

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πŸ“œ SIMILAR VOLUMES

Synthesis of L-[35S]homocysteine thiolac
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L-[ 35S]Homocysteine thiolactone has been synthesized by demethylation of L-{ 35S]Methionine with sodium in liquid ammonia and subsequent lactonisation in acid solution. The radiochemical yield of the carrier added synthesis was in the range of 45 to 50 % with a radiochemical purity higher than 96 %

Preparation of 35S-labelled l-methionine
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✍ S. R. Albahari; M. B. Skakun-Todorovi πŸ“‚ Article πŸ“… 1978 πŸ› John Wiley and Sons 🌐 French βš– 495 KB

## Abstract The modified method for biosynthesis of L‐methionine‐^35^S and L‐cystine‐^35^S from baker's yeast (__Saccharomyces cerevisiae__) is described. The yeast was cultivated in a sulphur‐deplated medium containing carrier‐free Na~2~^35^SO~4~, under air bubbling during 24 hours. Yeast proteins