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The polymerase chain reaction

✍ Scribed by Thomas J. White; Norman Arnheim; Henry A. Erlich

Book ID: 113288799
Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 567 KB
Volume: 5
Category: Article
ISSN: 0168-9525
DOI: 10.1016/0168-9525(89)90073-5

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A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kin