Polymerase chain reaction engineering
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James T. Hsu; Simantini Das; Satish Mohapatra
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Article
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1997
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John Wiley and Sons
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English
β 201 KB
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A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kin