Molecular biology: The polymerase chain reaction
β Scribed by Norris K. Lee
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 413 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1043-3074
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A DNA fragment longer than 10 kb can be amplified by the long and accurate polymerase chain reaction (LA-PCR) method. We demonstrate here applications of this technique in molecular biological studies of Saccharomyces cerevisiae. We have shown that DNA fragments amplified by LA-PCR can be directly u
A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kin