A method was developed for sex determination in two parrot species (Amazona a. aestiva and Amazona amazonica). A protocol is described for direct PCR amplification from parrot whole blood using primers designed for Xho I repetitive regions of chicken W-chromosome. A Wspecific fragment (approximate s
Determination of the Rh factor: A practical illustrating the use of the polymerase chain reaction
β Scribed by Santiago Imperial; Albert Boronat
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2005
- Tongue
- English
- Weight
- 82 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1470-8175
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract We investigated whether the polymerase chain reaction (PCR) could be used to determine the mechanism of mutation in lymphocyte clones mutated at the HLAβA locus. Three polymorphisms, at Factor XIIIA, D6S109, and intron 3 of the HLAβA gene, were used to study a series of clones previousl
## BACKGROUND. Polymerase chain reaction (PCR) based assays are becoming more reliable, simpler, and faster alternatives to traditional Southern blot hybridization (SBH) analysis for the detection of clonal immunoglobulin heavy chain (IgH) gene rearrangements. However, a variety of technical approa