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Sex determination of parrotsAmazona a. aestivaandAmazona amazonicausing the polymerase chain reaction

✍ Scribed by De Mattos, Keiko Kusamura; Del Lama, Sílvia Nassif; Salles, Ana Rita Cherman; Francisco, Mercival Roberto; Medaglia, Adriana; Tomasulo, Alessandra Moreira; Carmo, Fausto Moreira Silva; Pereira, Jairo Marques Campos; De Oliveira, Marcos Antônio

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
240 KB
Volume
281
Category
Article
ISSN
0022-104X

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✦ Synopsis

A method was developed for sex determination in two parrot species (Amazona a. aestiva and Amazona amazonica). A protocol is described for direct PCR amplification from parrot whole blood using primers designed for Xho I repetitive regions of chicken W-chromosome. A Wspecific fragment (approximate size of 124 bp) was amplified only in six DNA-female samples. No amplification was observed in 19 male samples. Complete correspondence was observed between our sex identification results and those obtained with endoscopy, karyotype analyses, and behavioral observations.

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