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Polymerase chain reaction amplification applied to the determination of β-like globin gene cluster haplotypes

✍ Scribed by Millicent Sutton; Eric E. Bouhassira; Dr. Ronald L. Nagel


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
301 KB
Volume
32
Category
Article
ISSN
0361-8609

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Polymerase chain reaction amplification
✍ Laura Manca; Elena Cocco; Bruno Masala; Dr John G. Gilman 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB 👁 2 views

We report here the identification of a 4 bp deletion in the 'yT globln gene promoter by means of Fnu4HI digestion of DNA amplified by polymerase chain reaction (PCR). This deietlon has been prevlously associated with haplotype II p-thalassemia in Sardinia. This simple, non-radioactive procedure shou