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Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene

✍ Scribed by Laura Manca; Elena Cocco; Bruno Masala; Dr John G. Gilman

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
234 KB
Volume
35
Category
Article
ISSN
0361-8609

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✦ Synopsis

We report here the identification of a 4 bp deletion in the 'yT globln gene promoter by means of Fnu4HI digestion of DNA amplified by polymerase chain reaction (PCR). This deietlon has been prevlously associated with haplotype II p-thalassemia in Sardinia. This simple, non-radioactive procedure should facilitate the screening of various populations of normal and p-thalassemic subjects for this specific genetic alteration.

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