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Novel approach to quantitative polymerase chain reaction using real-time detection: Application to the detection of gene amplification in breast cancer

✍ Scribed by Ivan Bièche; Martine Olivi; Marie-Hélène Champème; Dominique Vidaud; Rosette Lidereau; Michel Vidaud

Publisher: John Wiley and Sons
Year: 1998
Tongue: French
Weight: 325 KB
Volume: 78
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19981123)78:5<661::aid-ijc22>3.0.co;2-i

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Polymerase chain reaction amplification

Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene

✍ Laura Manca; Elena Cocco; Bruno Masala; Dr John G. Gilman 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB

We report here the identification of a 4 bp deletion in the 'yT globln gene promoter by means of Fnu4HI digestion of DNA amplified by polymerase chain reaction (PCR). This deietlon has been prevlously associated with haplotype II p-thalassemia in Sardinia. This simple, non-radioactive procedure shou