The plasmin-catalyzed hydrolysis of Nα-CBZ-L-lysine p-nitrophenyl ester
✍ Scribed by Robert M. Silverstein
- Book ID
- 119032897
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 422 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0049-3848
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📜 SIMILAR VOLUMES
The catalytic properties of human urokinase have been investigated using a synthetic chromogenic substrate; a-CBZ-L-lysine-p-nitrophenyl ester (ZLNP). The enzymatic assay based on the rate of hydrolysis of ZLNP offers several advantages over other methods currently employed in different laboratories
An assay for human plasminogen using NU-CBZ-L-lysine p-nitrophenyl ester as the substrate is described. The procedure is performed by activating the sample with streptokinase for 5 min at pH 6.0 and 25", the substrate is added to this mixture and its hydrolysis is monitored spectrophotometrically at