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The plasmin-catalyzed hydrolysis of Nα-CBZ-L-lysine p-nitrophenyl ester

✍ Scribed by Robert M. Silverstein


Book ID
119032897
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
422 KB
Volume
3
Category
Article
ISSN
0049-3848

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📜 SIMILAR VOLUMES


The hydrolysis of α-CBZ-l-lysine-p-nitro
✍ Paolo Ascenzi; Alberto Bertollini; Daniela Verzili; Maurizio Brunori; Eraldo Ant 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 360 KB

The catalytic properties of human urokinase have been investigated using a synthetic chromogenic substrate; a-CBZ-L-lysine-p-nitrophenyl ester (ZLNP). The enzymatic assay based on the rate of hydrolysis of ZLNP offers several advantages over other methods currently employed in different laboratories

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✍ Robert M. Silverstein 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 426 KB

An assay for human plasminogen using NU-CBZ-L-lysine p-nitrophenyl ester as the substrate is described. The procedure is performed by activating the sample with streptokinase for 5 min at pH 6.0 and 25", the substrate is added to this mixture and its hydrolysis is monitored spectrophotometrically at