An assay for human plasminogen using NU-CBZ-L-lysine p-nitrophenyl ester as the substrate is described. The procedure is performed by activating the sample with streptokinase for 5 min at pH 6.0 and 25", the substrate is added to this mixture and its hydrolysis is monitored spectrophotometrically at
The assay of the bromelains using Nα-CBZ-l-lysine p-nitrophenyl ester and N-CBZ-glycine p-nitrophenyl ester as substrates
✍ Scribed by Robert M. Silverstein
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 395 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0003-2697
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The catalytic properties of human urokinase have been investigated using a synthetic chromogenic substrate; a-CBZ-L-lysine-p-nitrophenyl ester (ZLNP). The enzymatic assay based on the rate of hydrolysis of ZLNP offers several advantages over other methods currently employed in different laboratories
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