✦ LIBER ✦

The hydrolysis of α-CBZ-l-lysine-p-nitrophenyl ester by two forms of human urokinase

✍ Scribed by Paolo Ascenzi; Alberto Bertollini; Daniela Verzili; Maurizio Brunori; Eraldo Antonini

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 360 KB
Volume: 103
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90262-6

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✦ Synopsis

The catalytic properties of human urokinase have been investigated using a synthetic chromogenic substrate; a-CBZ-L-lysine-p-nitrophenyl ester (ZLNP). The enzymatic assay based on the rate of hydrolysis of ZLNP offers several advantages over other methods currently employed in different laboratories. The steady state parameters of the two purified forms of human urokinase, which differ in molecular weight (33,OOO and 54,OOO daltons), have been determined over the pH range 5.2-7.8, and found to be indistinguishable.

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