The phosphogluconate pathway and synthesis of 5-phosphoribosyl-1-pyrophosphate in human fibroblasts
โ Scribed by RAIVIO, K; LAZAR, C; KRUMHOLZ, H; BECKER, M
- Book ID
- 122675041
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 512 KB
- Volume
- 678
- Category
- Article
- ISSN
- 0304-4165
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๐ SIMILAR VOLUMES
The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr
The product of the enzyme 5-phosphoribosyl-1-pyrophosphate (PPriboseP) synthetase is a substrate for purine, pyrimidine, and pyridine biosynthesis and may be rate limiting for purine biosynthesis. A system developed to electrophoretically separate and histochemically detect PPriboseP synthetase in c