Electrophoretic heterogeneity of 5-phosphoribosyl-1-pyrophosphate synthetase within and among humans

✍ Scribed by Roger V. Lebo; David W. Martin

• Book ID: 104783682
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 598 KB
• Volume: 16
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00483742

✦ Synopsis

The product of the enzyme 5-phosphoribosyl-1-pyrophosphate (PPriboseP) synthetase is a substrate for purine, pyrimidine, and pyridine biosynthesis and may be rate limiting for purine biosynthesis. A system developed to electrophoretically separate and histochemically detect PPriboseP synthetase in crude cell extracts has facilitated the identification of electrophoretically variant enzyme forms in the erythrocytes of five patients from a patient population of 200. Additional studies of human organs obtained at autopsy revealed a unique electrophoretic mobility for nearly each organ within the same individual.