The mouse Ly-12.1 specificity: genetic and biochemical relationship to Ly-1

✍ Scribed by P. M. Hogarth; B. A. Houlden; S. E. Latham; M. Cherry; B. A. Taylor; I. F. C. McKenzie

• Book ID: 104754737
• Publisher: Springer-Verlag
• Year: 1988
• Tongue: English
• Weight: 399 KB
• Volume: 27
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00395135

✦ Synopsis

The Ly-12 locus was originally defined in a study in which six loci were detected using conventional alloantisera made by immunizing between strains C57L and 129 (Potter and McKenzie 1981). These antisera were difficult to analyze as they were complex and required extensive absorption to define each specificity and consequently were of low and variable titer. Thus, although the Ly-12 locus was defined, nothing was known of its chromosomal location, or chemistry, and its tissue distribution was also not completely defined.

We now describe a more complete analysis of the Ly-12 locus using a monoclonal antibody, 5084-4.1, produced after immunizing 129/ReJ mice weeny with 5 X 107 C57L/J thymocytes for 5 weeks. The 129/ReJ spleen cells were prepared and fused with P3-NSI-1-Ag4 cells as described (Hogarth et al. 1980). The 5084-4.1 cell line was isolated and secreted on IgG2a Ly-12.1-specific antibody that was cytotoxic in the presence of rabbit complement.