The B cell alloantigen Ly-17.1 is controlled by a gene closely linked to Ly-20 and Ly-9 on chromosome 1

✍ Scribed by Wendy F. Davidson; Herbert C. Morse; Bonnie J. Mathieson; Christine A. Kozak; Fung-Win Shen

• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 229 KB
• Volume: 17
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364416

✦ Synopsis

Ly-17.1 is an alloantigen expressed on B cells and bone marrow but not on peripheral T cells or thymocytes (Shen and Boyse 1980). Antibodies (anti-Ly-17.1) specific for this determinant are present in (C3Hf/Bi x B6-Tla a) anti-C3H/An spleen (Shen and Boyse 1980) and AKR/N anti-C3H/HeN thymocyte antisera (W. F. Davidson and B.J. Mathieson, unpublished data). These antibodies are not cytotoxic but they can be detected by the PA-SRBC rosetting assay of Koo and Goldberg (1978) or by flow microfluorometry (FMF) using fluorescein (F1)conjugated anti-IgG2 (W. F. Davidson and B.J. Mathieson, unpublished data). Using the PA-SRBC rosetting assay, it was shown that Ly-17.1 expression was controlled by a single semi-dominant gene that was not closely linked to certain loci on chromosomes 1, 4, 6, 17, 19, or the X chromosome (Shen and Boyse 1980). In the present communication, the genetic control of Ly-17.1 antigen expression was reexamined. Lymph-node cells from Nll backcross segregants bred to derive a congenic strain, B6.Ly-17.1 (allele donor C3H/An = Ly-9.1, Ly-17.1 +, Ly-20.2-) and spleen cells from (C57BL/6J x A/J)F 1 x NFS/N outcross mice were assayed by FMF for expression of Ly-17.